This study was conducted to recognize the physical and chemical properties of baobab seeds crude oil and its use in food frying process. The seeds were milled for proximate analysis: oil content, protein content, moisture content, total ash, crude fiber, and carbohydrate, then the results were recorded 27.5%, 14.0%, 5.7%, 3.4%, 15.0%, 34.4% respectively. The oil was extracted by mechanical pressing, then the physical properties density, viscosity, refractive index, and moisture content were tested, the results were recorded 0.9139, 22.6 cp, 1.4690, 0.1% respectively. Also the chemical properties peroxide value, free fatty acids, saponification value, and iodine value, were determined, the results were recorded 9.0 meqo2/kg, 1.8%, 240.4, 54.0 respectively.